DNA-binding cationic liposomes are routinely employed as one of the major non-viral transfecting agents delivering DNA and other genes inside the cells. Cationic liposomes when complexed with DNA form a strong positively charged liposome-DNA complex or lipoplex. The chapter discusses primarily the major preparation technique for cationic liposomes and its physical characterization, with a focus on SYBR Green-I dye exclusion assay and DNA encapsulation enhancement by freeze-thaw technique. SYBR Green-I dye exclusion assay is a technique to determine the total amount of liposomal lipids required to bind a unit weight of DNA, which is critical for transfection experiments. Freeze-thaw technique on the other hand is one of the major techniques to improve DNA encapsulation in cationic liposomes.
CITATION STYLE
Saxena, V. (2017). Preparation and physical characterization of DNA-binding cationic liposomes. In Methods in Molecular Biology (Vol. 1522, pp. 245–250). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6591-5_19
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