The rapidly growing list of small RNA species generated by next-generation sequencing technologies has accelerated the development of new bioinformatics tools for their detection. Small RNAs generated from tRNAs, transfer RNA-derived fragments (tRFs), represent a novel challenge in accurately identifying and distinguishing them from random degradation products of tRNAs. Here, we describe a bioinformatics approach to detect tRFs in next-generation sequencing libraries. We also present a biochemical purification protocol for enriching 5′ tRFs and separating them from miRNAs. And finally, we suggest reliable methods for detecting and quantifying tRFs.
CITATION STYLE
Keam, S. P., Sobala, A., Humphreys, D. T., Suter, C. M., & Hutvagner, G. (2014). Computational analysis, biochemical purification, and detection of tRNA-derived small RNA fragments. Methods in Molecular Biology, 1173, 157–167. https://doi.org/10.1007/978-1-4939-0931-5_14
Mendeley helps you to discover research relevant for your work.