Rapid miniprep of DNA from filamentous fungi.

Abstract

Routine screening of a large number of transformants by Southern of slot blot analyses requires rapid DNA isolation from small fungal cultures. The authors have developed a procedure which allows the easy preparation of DNA from 100 or more isolates per day. This technique has been used successfully with Asperguillus nidulans, A. niger, Penicillium chrysogenum and Neurospora crassa