Plasmacytoid dendritic cells (pDCs) constitute a unique DC subset specialized in rapid and massive secretion of cytokines, including type I interferon (i.e., IFNα and IFNβ), known to be pivotal for both innate immunity and the onset of adaptive response. The production of type I IFNs by pDCs is primarily induced by the recognition of viral nucleic acids through Toll-like receptor (TLR)-7 and -9 sensors located in the endolysosomal compartment. Importantly, in the context of hepatitis C virus (HCV) infection, pDC type I IFN response is triggered by the sensing of infected cells via physical cell–cell contact. Such a feature is also observed for many genetically distant viruses, including notably viruses of the Retroviridae, Arenaviridae, Flaviviridae, Picornaviridaea, Togaviridae families and observed for various infected cell types. Here, we described a set of experimental methods for the ex vivo studies of the regulation of pDC activation upon physical cell–cell contact with virally infected cells.
CITATION STYLE
Coléon, S., Assil, S., & Dreux, M. (2019). Monitoring of interferon response triggered by cells infected by hepatitis C virus or other viruses upon cell–Cell contact. In Methods in Molecular Biology (Vol. 1911, pp. 319–335). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8976-8_22
Mendeley helps you to discover research relevant for your work.