Effects of Temperature on 6-O-α-Maltosyl Cyclodextrin Production from α-Maltosylfluoride and Cyclodextrins

Abstract

The effects of temperature on 6-O-α-maltosyl cyclodextrins (G2-CDs) production from α-maltosylfluoride (α-G2F) and cyclodextrins (CDs) by the transfer action of debranching enzymes such as pullulanase and isoamylase were studied. The amounts of 6-O-α-maltosyl α-cyclodextrin (G2-α-CD) production by purified pullulanase from Aerobacter aerogenes (A-pullulanase) and from Bacillus acidopullulyticus (B-pullulanase) increased with a rise in temperature, e.g., the amounts at 60°C were about 1.5 times higher than those at 30°C. Initial transfer ratios (G2-α-CD formed/α-G2F consumed) of A-pullulanase and B-pullulanase were about 62% and 25% (at 40°C), and about 50% and 15% (at 20°C), respectively. The transfer ratios of both A-pullulanase and B-pullulanase in the reaction using β-CD or γ-CD as acceptor also increased with a rise in temperature. The transfer ratios were little affected by any change in temperature or any kind of acceptor CDs, in the case of isoamylase, and were about 60%. © 1988, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.