Natural compounds have generated great interest as alternative treatments of diseases like cancer. Here, we investigated the anti-tumor mechanism of one such compound, Agrocybe aegerita polysaccharide, by assessing expression of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in rat esophageal carcinoma (EC). EC was induced in healthy Wistar rats by methyl-n-amyl nitrosamine. Subsequently, rats were administered cancer treatment daily for 4 weeks, as follows: the normal control group (the only group not treated with methyl-n-amyl nitrosamine) and model group received only distilled water; the chemotherapy group received tegafur treatment; and the combination group received tegafur combined with A.aegerita polysaccharide. In normal and combination groups, body weight increased gradually after each week of treatment (P < 0.05), while body weights did not change in model and chemotherapy groups. Using enzyme linked immunosorbent assay, we found serum TNF-α was lower in the combination group (31.56 ± 7.20pg/L) than either the model (46.24 ± 8.52pg/L) or chemotherapy (52.39 ± 9.16pg/L) group, and, while higher, was more similar to the normal controls (25.08 ± 2.93pg/L; P < 0.05), a finding that was confirmed by the immunohistochemistry of esophageal samples. In contrast, serum IFN-γ was higher in the combination group (97.20 ± 10.92pg/L) than in either the model (76.11 ± 11.92pg/L) or chemotherapy (76.04 ± 9.85pg/L) group, but lower than in the normal group (117.56 ± 10.88; P < 0.05), also confirmed by immunohistochemistry. Therefore, Agrocybe aegerita polysaccharide, when combined with chemotherapy, can regulate immune function in EC, potentially by modulating cytokine activity, specifically downregulation of TNF-α and upregulation of IFN-γ. © 2012 Copyright the Authors. Journal compilation © 2012, Wiley Periodicals, Inc.
CITATION STYLE
Ji, Y., Zheng, M. F., Ye, S. G., Wu, X. B., & Chen, J. Y. (2013). Agrocybe aegerita polysaccharide combined with chemotherapy improves tumor necrosis factor-α and interferon-γ levels in rat esophageal carcinoma. Diseases of the Esophagus, 26(8), 859–863. https://doi.org/10.1111/j.1442-2050.2012.01397.x
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