Reaction volume and pressure effect in protein reactions

Abstract

In this article, reaction volumes and pressure effects on protein reactions are discussed by means of the pulsed laser induced transient grating (TG) spectroscopy. By the TG method, temporal profile of the protein reaction volume has been determined for the non-reversible reaction process. For examples, TG studies on the photo-chemical process of carboxymyoglobin and photoactive yellow protein are introduced. The reaction volume for each process has been determined, which can not be studied by the other spectroscopic methods such as transient absorption method. A high pressure optical system, which enabled us to measure the pressure dependence of the reaction volume, has also been reported.