Ribosome display for rapid protein evolution by consecutive rounds of mutation and selection

Abstract

Directed evolution experiments are performed to improve the properties of proteins by creating a library of mutated genes of interest and selecting those genes that encode proteins exhibiting desired properties. Here, we present one of the methods to carry out an evolutionary experiment called ribosome display. Ribosome display allows this process to be carried out entirely in vitro, and it is therefore a rapid and robust method for protein evolution. © 2010 Springer Science+Business Media, LLC.