Concentration of Sinigrin from Indian mustard (Brassica Juncea L.) seeds using nanofiltration membrane

Abstract

Sinigrin has drawn more attention of pathologists and immunologists, which exhibits a wide range of biological activities. In the present study, the feasibility of concentration of sinigrin using nanofiltration membranes was studied using one-factor-at-a-time method with the permeate flux and sinigrin retention as responses. After seven commercial membranes were compared, including NF, NF-270, SPESI, STARMEN 120, STARMEN 128, STARMEN 220, STARMEN 240, we investigated the effects of temperature, pressure, and feed concentration on the concentration process of sinigrin from crude aqueous extracts of Indian mustard (Brassica juncea L.) seeds followed by primary ultrafiltration. The results indicated that NF-270 membrane showed the best concentration capability to sinigrin. Their ranges of permeate flux and sinigrin retention were 7.44–23.47 L/(m 2 h) and 90.77–97.13%, respectively. Furthermore, the high flux and high retention were explained by swelling ratio, SEM, and ATR-FTIR, which were applied to determine the differences of nanofiltration membrane before and after soaking in aqueous solution. Finally, it could be concluded that the concentration process using nanofiltration provided a novel, rapid, and economical method for concentration of sinigrin.