Abstract
This chapter details a compendium of protocols that collectively enable the reader to perform a pooled shRNA and/or CRISPR screen—with methods to identify and validate positive controls and subsequent hits; establish a viral titer in the cell line of choice; create and screen libraries, sequence strategies, and bioinformatics resources to analyze outcomes. Collectively, this provides an overarching resource from the start to finish of a screening project, making this technology possible in all laboratories.
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Cluse, L. A., Nikolic, I., Knight, D., Madhamshettiwar, P. B., Luu, J., Cowley, K. J., … Simpson, K. J. (2018). A comprehensive protocol resource for performing pooled shRNA and CRISPR screens. In Methods in Molecular Biology (Vol. 1725, pp. 201–227). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7568-6_17
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