Spider venom contains a complex mixture of components with a large range of molecular masses (0.1-60 kDa) exhibiting a diverse array of actions. Most of these components are proteinaceous molecules - biologically active proteins and peptides. Proteomics profiling of spider venoms (the components with MW >10 kDa) could be achieved through conventional 2-DE-based proteomics methods combined with MS or MS/MS detection. Peptidomic profiling (of the components with MW below ~10 kDa) is usually achieved through off-line separation by a combination of ion-exchange and reverse-phase chromatography, and it relies more heavily on de novo sequencing by Edman degradation or MS/MS for peptide identification.
CITATION STYLE
Songping, L. (2010). Protocols for peptidomic analysis of spider venoms. Methods in Molecular Biology (Clifton, N.J.), 615, 75–85. https://doi.org/10.1007/978-1-60761-535-4_6
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