Gene functional studies consist of phenotyping cells with altered gene expression. Improving the precision of current gene expression control techniques would enable more detailed studies of gene function. Here, we provide protocols for building synthetic gene constructs for tuning the expression of a gene in all the cells of a population precisely and uniformly, achieving expression levels proportional to the extracellular inducer concentration.
CITATION STYLE
Nevozhay, D., Adams, R. M., & Balázsi, G. (2011). Linearizer Gene Circuits with Negative Feedback Regulation. In Methods in Molecular Biology (Vol. 734, pp. 81–100). Humana Press Inc. https://doi.org/10.1007/978-1-61779-086-7_5
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