Genomics on Pretreatment Inhibitor Tolerance of Zymomonas mobilis

Abstract

The development and use of robust ethanologenic microorganisms resistant to industrially relevant pretreatment inhibitors will be a critical component in the successful generation of biofuel on the industrial scale. Recent progress to understand the genetic basis of pretreatment inhibitor tolerance using genomics and systems biology tools for metabolic engineering for the model ethanologenic bacterium Zymomonas mobilis is reviewed in this chapter. The importance of accurate genome annotations and the integration of systems biology data for annotation improvement are highlighted, and case studies that describe the identification and characterization of the Z. mobilis nhaA, hfq, and himA inhibitor tolerance related gene targets are presented.