Utilization of polyfloral honey in the synthesis of gold nanoparticles and evaluation of its potency as an antibacterial against S. aureus and E. coli

Abstract

Gold nanoparticles was synthesized undergoes a green synthesis by utilising polyfloral honey as a bioreducing agents. Resulted gold nanoparticles were characterized using UV-Vis and Fourier Transform Infra Red (FTIR) spectrometer, as well as X-Ray Diffraction (XRD) and Particle Size Analyser (PSA). Spectroscopy analysis of obtained products gave the results of an absorbance of 1.095 at wave length of 543 nm for UV-Vis, while Fourier Transform Infra Red (FTIR) shown the appearance of certain type of organic functional group, which are characteristic for sugars, the major constituents of polyfloral honey, such as O-H, C=O, N-H and C-O in the gold nanoparticle synthesis. Analysis of XRD resulted of the Miller Index 111, 200, 202 and 311 of the product that corresponding to the database of Joint Committee on Powder Diffraction Standards (JCPDS). Particle Size Analyzer result average size distribution of gold nanoparticle is 219,8 nm. The evaluation of test antibacterial activity was performed using several bacteria test such as S. aureus and E. coli that show the gold nanoparticles can inhibit the bacteria.