Genetic reporters offer attractive approaches to investigate gene expression, gene function, and spatiotemporal assessment in vitro and in vivo. Tyrosine hydroxylase (TH) is the rate-limiting enzyme for the biosynthesis of the dopamine neurotransmitter, and thus it has been used as a reliable marker for dopaminergic neurons in vitro and in vivo. Herein we describe a method for making iPSC lines with TH-green fluorescent protein reporter gene using CRISPR/Cas9 technique.
CITATION STYLE
Hong, H., & Daadi, M. M. (2019). Generating neural stem cells from iPSCs with dopaminergic neurons reporter gene. In Methods in Molecular Biology (Vol. 1919, pp. 119–128). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9007-8_9
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