Flux analysis is performed to infer intracellular metabolic activity using measured rates. By applying the highly curated human metabolic reconstruction Recon 2 as the reference model, the investigation of cancer cell metabolic fluxes can encompass the full metabolic potential of a human cell. But in its full form, Recon 2 is unsuitable for conventional metabolic flux analysis due to a large number of redundant elements. Here, we describe a procedure to reduce Recon 2 to an appropriate scale for cancer cell flux analysis such that calculated flux intervals are still informative, without compromising the opportunity to explore alternative pathways encoded in Recon 2 that may reveal novel metabolic features.
CITATION STYLE
Quek, L. E., & Turner, N. (2019). Using the human genome-scale metabolic model recon 2 for steady-state flux analysis of cancer cell metabolism. In Methods in Molecular Biology (Vol. 1928, pp. 479–489). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9027-6_25
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