Development of gateway binary vectors R4L1pGWB possessing the bialaphos resistance gene (bar) and the tunicamycin resistance gene as markers for promoter analysis in plants

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Abstract

We have developed a new series of R4L1 Gateway binary vectors (R4L1pGWB), which carry the bialaphos resistance gene (bar) or the UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as selection markers that confer BASTA® and tunicamycin resistance on plants respectively. R4L1pGWBs have an attR4-attL1-reporter and can accept an attL4-promoter-attR1 entry clone for easy construction of an attB4-promoter-attB1-reporter clone. The new R4L1pGWBs facilitate promoter:reporter analysis in pre-existing transgenic plants that are resistant to kanamycin or hygromycin.

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Tanaka, Y., Shibahara, K., & Nakagawa, T. (2013). Development of gateway binary vectors R4L1pGWB possessing the bialaphos resistance gene (bar) and the tunicamycin resistance gene as markers for promoter analysis in plants. Bioscience, Biotechnology and Biochemistry, 77(8), 1795–1797. https://doi.org/10.1271/bbb.130405

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