We have developed a polymerase chain reaction (PCR) assay which distinguishes genotype F from the other genotypes of hepatitis B virus (HBV). The method was used to characterize HBV strains isolated in urban areas of the Brazilian Amazon. DNA was amplified in 54 of a total of 78 HBsAg-positive serum samples, using universal, non-genotype-specific primers. Only 4 (7.4%) were identified as genotype F by our genotype-specific PCR assay. This proportion is notably lower than that previously reported in Argentina, Venezuela, Peru, and Central America.
CITATION STYLE
Moraes, M. T. B., Niel, C., & Gomes, S. A. (1999). A polymerase chain reaction-based assay to identify genotype F of hepatitis B virus. Brazilian Journal of Medical and Biological Research, 32(1), 45–49. https://doi.org/10.1590/S0100-879X1999000100006
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