C. chauvoei presence was detected by means of polymerase chain reaction (PCR) from supernatant of culture in cooked meat medium of liver, muscle and metatarsian bone marrow samples of seven calves with blackleg symptoms. The isolation under anaerobic conditions of one muscle sample revealed Clostridium perfringens in pure culture.
CITATION STYLE
Miyashiro, S., Nassar, A. F. C., Souza, M. C. A. M., Carvalho, J. B., & Adegas, J. E. B. (2007). Identification of Clostridium chauvoei in clinical samples cultures from blackleg cases by means of PCR. Brazilian Journal of Microbiology, 38(3), 491–493. https://doi.org/10.1590/S1517-83822007000300020
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