Treatment with Recombinant Bovine Interferon-τ in Utero Attenuates Secretion of Prostaglandin F from Cultured Endometrial Epithelial Cells

Abstract

Endometrial oxytocin receptors and total production of PGF by endometrial epithelial cells were measured in 10 cyclic cows after intrauterine injections of recombinant bovine interferon-τ plus BSA or BSA alone. Cows received twice daily injections (via intrauterine catheters) of 200 μg of recombinant bovine interferon-τ plus 1.3 mg of BSA (n = 5) or 1.5 mg of BSA (n = 5) from d 14 to 17 after estrus. On d 17, the reproductive tracts of each cow was removed at slaughter, and endometrial epithelial cells were cultured with 0, 2, or 50 ng/ml of recombinant bovine interferon-τ. After 24 h, oxytocin (2 × 10-7 M) was added to one-half of the culture wells, and the medium was sampled at 0, 30, and 90 min for analysis of total PGF (PGF plus 13,14-dihydro-15-keto-PGF2α). In vivo treatment with recombinant bovine interferon-τ + BSA reduced total secretion of PGF in culture (1.49 ± 0.06 vs. 2.80 ± 0.07 ng/μg of DNA), but did not block the oxytocin-induced stimulation in total secretion of PGF. In vitro treatment of cells with recombinant-bovine interferon-τ did not decrease basal secretion of total PGF. Oxytocin receptor binding at d 17 was low in both treatments but slightly attenuated in the group treated with recombinant bovine interferon-τ.