Transient expression is a powerful method for the functional characterization of genes. In this chapter, we outline a protocol for the transient expression of constructs in Medicago truncatula leaves using Agrobacterium tumefaciens infiltration. Using quantitative real-time PCR we demonstrate that the infiltration of a construct containing the LEGUME ANTHOCYANIN PRODUCTION 1 (LAP1) transcription factor results in the strong upregulation of key biosynthetic genes and the accumulation of anthocyanin pigment in the leaves after just 3 days. Thus, this method provides a rapid and powerful way to the discovery of downstream targets of M. truncatula transcription factors. © 2013 Springer Science+Business Media, LLC.
CITATION STYLE
Picard, K., Lee, R., Hellens, R., & Macknight, R. (2013). Transient gene expression in Medicago truncatula leaves via agroinfiltration. Methods in Molecular Biology, 1069, 215–226. https://doi.org/10.1007/978-1-62703-613-9_15
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