We propose a prediction method for human full-length cDNA by comparing sequence data between human genome shotgun sequence and mouse full-length cDNA. The human genome which is homologous to the mouse full-length cDNA is selected by a homology search program, and the predicted exons are connected at the exon-intron junction which gives the best homology score to the mouse full-length cDNA. The accuracy of the predicted human full-length coding region is 83.3%, and the false positive rate is 16.7%. Five human full-length proteins out of 20 proteins are correctly predicted.
Fukunishi, Y., Suzuki, H., Yoshino, M., Konno, H., & Hayashizaki, Y. (1999). Prediction of human cDNA from its homologous mouse full-length cDNA and human shotgun database. FEBS Letters, 464(3), 129–132. https://doi.org/10.1016/S0014-5793(99)01696-8