Decellularization is the process of removal of native cells from tissue, leaving behind a three-dimensional (3D) ultrastructure of extracellular matrix (ECM) proteins while preserving the bioactivity and mechanics of the tissue. It offers a unique top-down approach for fabricating ECM based natural scaffold for tissue engineering application. Herein, this chapter presents the fabrication of decellularized scaffold employing different methods: Whole organ perfusion, immersion and agitation, pressure gradient, and supercritical fluid. The decellularized scaffold aims to exploit the nature-designed 3D architecture, a successful platform technology, for creating scaffolding materials for tissue engineering and regenerative medicine.
CITATION STYLE
Gupta, S. K., Mishra, N. C., & Dhasmana, A. (2018). Decellularization methods for scaffold fabrication. In Methods in Molecular Biology (Vol. 1577, pp. 1–10). Humana Press Inc. https://doi.org/10.1007/7651_2017_34
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