The advent of new DNA sequencing technologies leads to a dramatic increase in the number of available genome sequences and therefore of target genes with potential for functional analysis. The insertion of these sequences into proper expression vectors requires a simple an efficient cloning method. In addition, when expressing a target protein, quite often it is necessary to evaluate different DNA constructs to achieve a soluble and homogeneous expression of the target with satisfactory yields. The development of new molecular methods made possible the cloning of a huge number of DNA sequences in a high-throughput manner, necessary for meeting the increasing demands for soluble protein expression and characterization. In this chapter several molecular methods suitable for high-throughput cloning are reviewed.
CITATION STYLE
Ortega, C., Abreu, C., Oppezzo, P., & Correa, A. (2019). Overview of high-throughput cloning methods for the post-genomic era. In Methods in Molecular Biology (Vol. 2025, pp. 3–32). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9624-7_1
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