In previous reports, hesperidin, a flavonoid glucoside from citrus fruit, is hydrolyzed to hesperetin, an aglycone of hesperidin, and converted to the hesperetin glucuronides (H7-OG and H3′-OG) in vivo and depresses blood glucose levels. But there are no reports on the activity of hesperetin glucuronides. To determine the activity of hesperetin glucuronides, H7-OG and H3′-OG were synthesized and peroxisome proliferator-activated receptor-γ (PPARγ) agonist activity was observed at 250 μM. These glucuronides accelerated the differentiation of 3T3- L1 cells into adipocytes at 10 μM. Furthermore, H7- OG showed additive effects in reporter gene assays and caused noncompetitive reactions in timeresolved fluorescence resonance energy transfer assays with a thiazolidinedione derivative. Our results indicated that hesperetin glucuronides activated PPARγ, accelerated adipocyte differentiation.
CITATION STYLE
Gamo, K., Miyachi, H., Nakamura, K., & Matsuura, N. (2014). Hesperetin glucuronides induce adipocyte differentiation via activation and expression of peroxisome proliferator-activated receptor-γ. Bioscience, Biotechnology and Biochemistry, 78(6), 1052–1059. https://doi.org/10.1080/09168451.2014.910097
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