Laser microdissection and saturation labeling dige method for the analysis of human arteries

Abstract

Laser microdissection (LMD) is a novel methodology for noncontact isolation of tissue regions or cells for subsequent molecular analysis. Although it is an upcoming fi eld, its combination with proteomics for differential analysis remains not very well explored, since amount of protein obtained after LMD is scarce. We have combined LMD arterial layer isolation with saturation labeling DIGE, successfully achieving differential analysis of healthy and pathological intima and media layers. Identi fi cation of differential spots could be performed in whole tissue extract as reference proteome, since studied regions are subproteomes of the aforementioned. © 2013 Springer Science+Business Media New York.