Abstract
We present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presented. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.
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Heilemann, M., Hwang, L. C., Lymperopoulos, K., & Kapanidis, A. N. (2009). Single-molecule FRET analysis of protein-DNA complexes. Methods in Molecular Biology, 543, 503–521. https://doi.org/10.1007/978-1-60327-015-1_29
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