Redox responses in patients with sepsis: High correlation of Thioredoxin-1 and macrophage migration inhibitory factor plasma levels

Abstract

Background. Redox active substances (e.g., Thioredoxin-1, Macrophage Migration Inhibitory Factor) seem to be central hubs in the septic inflammatory process. Materials and Methods. Blood samples from patients with severe sepsis or septic shock (n=15) were collected at the time of sepsis diagnosis (t0), and 24 (t24) and 48 (t48) hours later; samples from healthy volunteers (n=18) were collected once; samples from postoperative patients (n=28) were taken one time immediately after surgery. In all patients, we measured plasma levels of IL-6, TRX1 and MIF. Results. The plasma levels of MIF and TRX1 were significantly elevated in patients with severe sepsis or septic shock. Furthermore, TRX1 and MIF plasma levels showed a strong correlation (t0: r sp =0.720,=0.698/t24: r sp =0.771,=0.949). Conclusions. Proinflammatory/~oxidative and anti-inflammatory/ ~oxidative agents show a high correlation in order to maintain a redox homeostasis and to avoid the harmful effects of an excessive inflammatory/oxidative response. © 2010 Thorsten Brenner et al.