Abstract
Traditional Chinese medicines (TCMs) serve as a major source of a variety of drug lead compounds. In the process of natural products development, bioassay-guided isolation is a rapid and validated method for isolation of compounds with bioactivities. This chapter describes bioassay-guided separation and purifi cation of compounds from the crude extracts of TCMs. Two approaches including size-exclusion chromatography (SEC) and high performance liquid chromatography (HPLC) are described in detail.
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Han, J., Zhang, J., He, W., Huang, P., Oyeleye, A., Liu, X., & Zhang, L. (2015). Bioassay-guided identification of bioactive molecules from traditional Chinese medicines. Methods in Molecular Biology, 1263, 187–196. https://doi.org/10.1007/978-1-4939-2269-7_15
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