Pulse Field Gel Electrophoresis (PFGE) is a powerful genotyping technique used for the separation of large DNA molecules (entire genomic DNA) after digesting it with unique restriction enzymes and applying to a gel matrix under the electric field that periodically changes direction. PFGE is a variation of agarose gel electrophoresis that permits analysis of bacterial DNA fragments over an order of magnitude larger than that with conventional restriction enzyme analysis. It provides a good representation of the entire bacterial chromosome in a single gel with a highly reproducible restriction profile, providing clearly distinct and well-resolved DNA fragments.
CITATION STYLE
Sharma-Kuinkel, B. K., Rude, T. H., & Fowler, V. G. (2016). Pulse field gel electrophoresis. In Methods in Molecular Biology (Vol. 1373, pp. 117–130). Humana Press Inc. https://doi.org/10.1007/7651_2014_191
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