The effect of brain-derived neurotrophic factor on regeneration of nerve fibers after crush injury - Morphometric Evaluation

Abstract

Brain-derived neurotrophic factor (BDNF) is produced in Schwann cells and fibroblasts in the peripheral nerve, and is considered to play an important role in survival, maintenance, development and repair of the peripheral neuron. In this study, the effect of human recombinant BDNF on the regeneration of nerve fibers following a crush injury to the sciatic nerves of Sprague-Dawley rats was evaluated. In the experimental group, 20 mg/kg of BDNF was injected subcutaneously three times a week for 4 weeks in seven rats. In the control group, only the dissolving solution used in the experimental group was injected similarly in seven rats as in the experimental group. The various morphometric parameters were evaluated in the peroneal, sural and tibial nerves and the nerve to soleus muscle distal to the crush site. There were no statistically significant differences in the change of body weight, the improvement of the width between the first and fifth toes on the side of the crush, the density of total myelinated fibers (numbers per mm), the total number of myelinated fibers (number per nerve), maximum and median diameters of myelinated fibers, the density of unmyelinated fibers, and the electron microscopic parameters of axon and myelin sheath. On the other hand, the number of myelin lamellae in relation to the transverse axonal area was significantly greater in the experimental group than in the control group. Therefore, BDNF may promote the myelination. However, such an effect on myelination seems not to be clinically significant, because such an effect was not demonstrated in other morphometric evaluations reflecting the myelination condition. Therefore, taking all the data obtained in this study into consideration, we concluded that there was no definite evidence that BDNF promoted the regeneration of nerve fibers at least under these experimental conditions.