Mmu-circ-003795 regulates osteoblast differentiation and mineralization in MC3T3-E1 and MDPC23 by targeting COL15A1

Abstract

Circular RNA s (circRNA s) are a class of non-coding RNA s that exhibit important regulatory roles in various biological processes. However, the role of circRNA s and their potential role in osteoblast differentiation and mineralization is unclear. The aim of the present study was to investigate the expression of mmu-circ-003795 and its effect on collagen type XV α 1 chain (COL15A1). First, it was identified that the expression levels of mmu-circ-003795 and osteopontin (OPN) were upregulated in the induced cells. Silencing of mmu- circ-003795 reduced the gene and protein levels of COL 15A1 and OPN, whereas the expression level of mmu-microRNA (miR)-1249-5p was upregulated. In addition, after 7 or 14 days of induction, alkaline phosphatase and Alizarin Red-S staining were decreased in the mmu-circRNA -003795 inhibitory group compared with the negative control group. In conclusion, mmu-circ-003795 may regulate osteoblast differentiation and mineralization in MC3T3-E 1 and MDPC23 cells via mmu-miR- 1249-5p by targeting COL 15A1.