Paracoccus denitrificans strain PD1222 (from N. Harms, Vrije Universiteit, Amsterdam, The Netherlands), which is deficient in a host-specific restriction system, could be successfully used for (1) transposon Tn5 mutagenesis, (2) phage P1-mediated generalized transduction and (3) construction of Hfr strains. Experimental protocols and some properties of an ammonium transport-deficient mutant are described. © 1991.
CITATION STYLE
Ghozlan, H. A., Ahmadian, R. M., Fröhlich, M., Sabry, S., & Kleiner, D. (1991). Genetic tools for Paracoccus denitrificans. FEMS Microbiology Letters, 82(3), 303–306. https://doi.org/10.1111/j.1574-6968.1991.tb04899.x
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