Culturing and differentiating mouse embryonic stem cells

Abstract

Pluripotent embryonic stem (ES) cells have been used extensively for over 20 years for creating mouse mutants as models for developmental biology and humans diseases. The genetic manipulations of the ES cells have revolutionized our understanding of organ development and abilities to genetically manipulate the mouse embryo. Understanding the ES cell differentiation has provided new insights essential for establishing cell-based therapy and tissue regeneration.