Comparative Efficacy of Different Cryoprotectants for Deep Freezing of Buffalo Bull Semen

Abstract

Different cryo-protectants are commonly used to reduce the chances of sperm damage during freezing of semen. Comparative effects of three glycerol extenders A, B and C with 7% glycerol, anhydrous glycerol 5% and 7% respectively were studied on sperm motility, livability and livability index during the post freezing technique of buffalo bull semen at Semen Production Unit Quadrabad. The six Nilli–Ravi Buffalo bulls were divided into two Key Words: Glycerol, Bull groups on the basis of age, three into each group. In group I animals were selected from 3 to 5 and semen years of age and in group II above from 5 years of age. Motility percentages after thawing in three extenders A, B and C were 46.88 ± 7.27, 54.69±7.85 and 61.24± 9.64 for three extenders respectively for group I. For group II the motility percentage after thawing in three extenders A, B and C were 48.13± 5.27, 57.19±7.85 and 64.38± 9.64 for three extenders respectively. The Livability after thawing for three extenders A, B and C were 4.56±1.20, 5.17±1.20 and 6.61±1.16 hrs respectively for group I. For group II values livability for three extenders A, B and C were 4.39±2.20, 5.33± 1.33 and 6.44±1.29 hrs respectively. Absolute index of livability for three extenders A, B and C averaged 136.83±35.27, 149.06±36.18 and 170.61±32.16 respectively for group I. For group II values of absolute index of livability for three extenders A, B and C were 125.61±25.27, 128.06±19.64 and 146.83±24.46 respectively. It was concluded that the extender C (containing 7 % anhydrous glycerol) showed best results in terms of motility after thawing and livability at 37°C followed by extender B ( containing 5 % anhydrous glycerol) and extender A (containing 7% glycerol)