Protein phosphorylation plays critical roles in multiple aspects of cellular events. Site- and phosphorylation state-specific antibodies are indispensable to analyze spatially and temporally distribution of protein phosphorylation in cells. Such information provides some clues of its biological function. Here, we describe a strategy to design a phosphopeptide as an antigen for a site- and phosphorylation state-specific antibody. Importantly, this strategy is also applicable to the production of other types of antibodies, which specifically recognize the site-specific modification, such as acetylation, methylation, and proteolysis. This protocol also focuses on the screening for monoclonal version of a site- and phosphorylation state-specific antibody. © 2014 Springer Science+Business Media, New York.
CITATION STYLE
Goto, H., & Inagaki, M. (2014). Method for the generation of antibodies specific for site and posttranslational modifications. Methods in Molecular Biology, 1131, 21–31. https://doi.org/10.1007/978-1-62703-992-5_2
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