Magnetic-activated cell sorting (MACS) is the technology that is recently used as a magnetic-based cell isolation/purification technique. This technique enables the isolation and selection of germ, hematopoietic, and somatic stem cells including skin stem cells (SkSCs). Here, we have tried to describe the isolation of stem cells by MACS using CD34 antigen for SkSCs, again CD34 for hematopoietic stem cells (HSCs) and Thy-1 for spermatogonial stem cells (SpSCs). MACS allowed the isolation of CD34+, CD34+, and Thy-1+ human SkSCs, HSCs, and SpSCs with minimum 98% purity.
CITATION STYLE
Korkusuz, P., Köse, S., Yersal, N., & Önen, S. (2019). Magnetic-based cell isolation technique for the selection of stem cells. In Methods in Molecular Biology (Vol. 1879, pp. 153–163). Humana Press Inc. https://doi.org/10.1007/7651_2018_151
Mendeley helps you to discover research relevant for your work.