Comparative evaluation of biochemical profiling of 4', 4"- (4, 5, 6, 7-TETRAHYDRO- [1, 2, 3-] SELENADIAZOLO [4, 5e] PYRIDINE-4, 6-DIYL) BIS (BENZENE-1, 3-DIOL) and dipyrone on female rat's

Abstract

The current study has been carried out at the department of pharmaceutical chemistry, College of pharmacy. Novel synthetically selenium-containing compounds have potential therapeutic effects towards several diseases, such as: cancer microbial infections and neurodegenerative diseases. Therefore, the present study accentuated mainly on two significant items. A novel selenadiazole derivative i.e, 4', 4"- (4, 5, 6, 7-TETRAHYDRO- [1, 2, 3-] SELENADIAZOLO [4, 5e] PYRIDINE-4, 6-DIYL) BIS (BENZENE-1, 3-DIOL) (T) and Dipyrone (Di) were used to detretmine their Biochemical effects on female rats. Biochemical test including; liver function tests; Renal functions tests; in addition lipid profile. Invivo study conducted using four groups, one as control (DW) and three treated groups (T, Di, and T&Di). The rats received 50mg/kg body weight (BW) of one of test treatments T and/or Di dissolved in 2 milliliter of distilled water and control group received same volume of distilled water for 30 days. Blood sample were collected directly from the rats heart under chloroform effect. The results indicated that Liver function test showed following results; Aspartate aminotransferase levels(AST) measurement it was cleared that only (T&Di) group (87.52 U/L ±12.20) was increased significantly than both DW(57.23 U/L ±10.43) and T(57.62 U/L ±16.54) groups. Alanine transaminase (ALT) concentration measurements showed only (T&Di) group (70.11 U/L ±13.09) value increased significantly than (DW), (T), (Di) groups. Alkaline Phosphatase (ALP) value of Di group (128.24 U/L ±27.9) highly elevated than in DW (66.68 U/L±15.29) and other test groups. Total protein (TP) concentrations of (Di) (4.97g/dL±1.02), (T) (10.87 g/dL ±3.25) and (T&Di) (5.05 g/dL ±0.76) groups highly reduced than (DW) group (14.80 g/dL ±1.98) level. Lipid profile test results show significant increase of Cholesterol (TC) level of (T) group (533.8mg/dL±52.5) than both DW (335.8mg/dL±27.01) and (T&Di) (390.3mg/dL±25.8) groups. Triglyceride (TG) serum levels only (T) group (100.1 mg/dL ±9.1) showed a significant reduction of TG value than in (Di) group (221.0572mg/dL ±39.8). Levels of HDL of (T) treated group (337.9 mg/dL±26.6) significant increased than all groups. VLDL levels results showed only Di group (43.4mg/dL±4.3) increased significantly than DW group; however there was significant decreased of T group VLDL level (20.03mg/dL±1.8) compare with Di (43.4mg/dL±4.3) and T&Di (35.9mg/dL±3.7) levels. Renal function data reveals significant reduction in blood urea levels of (T) (5.471 mg/dL ±3.745) and (T&Di) (10.633±5.431). Serum uric acid values showed significant decline of (T) group (2.601±0.743) than DW group (5.515±2.046). Also, the results of the present study illustrate only Di treated group (2.33±0.209) had essential increased of Creatinine values than all other study groups. The study concluded that synthesized novel selenadiazole derivative, and Dipyrone have mild effects on liver, kidney, and lipid profile. However, the companion of both drugs has some of undesirable effects.