We describe a simple assay for studying and conditioning olfactory behaviors of adult zebrafish. The apparatus consists of a circular flow-through tank into which odorants can be administered in a controlled fashion. Odorants (conditioned stimuli; CS) are repeatedly paired with food flakes (unconditioned stimuli; UCS) that are provided inside a tethered floating feeding ring. In response to conditioning, zebrafish develop an odorant-dependent place preference and restrict appetitive swimming behavior to the vicinity of the feeding ring. This robust assay can also be conducted with groups of zebrafish and thus provides a potentially important tool for large behavioral screens. © 2011 Springer Science+Business Media, LLC.
CITATION STYLE
Braubach, O. R., Wyeth, R. C., Murray, A., Fine, A., & Croll, R. P. (2011). A simple and effective method to condition olfactory behaviors in groups of zebrafish. Neuromethods, 51, 85–97. https://doi.org/10.1007/978-1-60761-953-6_7
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