A Novel Three-Dimensional Cell Culture Method to Analyze Epidermal Cell Differentiation In Vitro

Abstract

Studies of the epidermis have been carried out in various models, such as the monolayer culture in vitro model and three-dimensional (3D) skin models, that are spatially organized to display the architectural features seen in human skin. These models have furthered our understanding of epidermal cell biology and provided quite a few lines of evidence on proliferation, cellular metabolism, morphological status, and state of differentiation. In this chapter, we describe a novel method using epithelial cell aggregates embedded in a collagen gel, instead of individual cells, for building cell-cell and cell-matrix interactions. Analyzing cell behaviors during epidermal differentiation would be helpful. Our method would help to analyze a possible regulatory mechanism underlying epidermal differentiation.