Graphical determination of dissociation constant, pKa of non-polar amino acids

Abstract

The dissociation constant (pKa) of non-polar amino acids including (alanine, glycine, valine phenylalanine and tryptophan) were determined by potentiometric titration technique. The pKa values obtained by extrapolation for alanine, glycine, and valine were 10.29, 9.87 and 9.91 respectively. The implications of the results are discussed and recommendations were made.