The study of dendritic cell involvement in complex phenomena that rely on multi-cellular interactions, such as immune homeostasis, stimulation, and tolerization, called for the investigation of dendritic cell functions within physiological context. To this end we have developed a conditional cell ablation strategy that is based on dendritic cell-restricted expression of a Diphtheria Toxin receptor (DTR) using the CD11c/Itgax promoter. Here, we provide basic protocols that describe the use of this prototypic dendritic cell ablation model and highlight pitfalls and strengths of the approach.
CITATION STYLE
Bar-On, L., & Jung, S. (2010). Defining in vivo dendritic cell functions using CD11c-DTR transgenic mice. Methods in Molecular Biology (Clifton, N.J.), 595, 429–442. https://doi.org/10.1007/978-1-60761-421-0_28
Mendeley helps you to discover research relevant for your work.