Ultracentrifugation, particularly the use of sucrose or cesium chloride density gradients, is a highly reliable and efficient technique for the purification of virus-like particles and protein cages. Since virus-like particles and protein cages have a unique size compared to cellular macromolecules and organelles, the rate of migration can be used as a tool for purification. Here we describe a detailed protocol for the purification of recently discovered virus-like assemblies called bacterial encapsulins from Thermotoga maritima and Brevibacterium linens.
CITATION STYLE
Rurup, W. F., Cornelissen, J. J. L. M., & Koay, M. S. T. (2015). Recombinant expression and purification of “virus-like” bacterial encapsulin protein cages. Methods in Molecular Biology (Clifton, N.J.), 1252, 61–67. https://doi.org/10.1007/978-1-4939-2131-7_6
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