Synthesis and characterization of hydroxyapatite-chitosan composite in situ by microwave irradiation method

Abstract

Chitosan is a natural polysaccharide which has bioresorbable, biocompatible, and non-toxic properties. The combination between chitosan and hydroxyapatite will form a nanocomposite which improves its mechanical properties and can be applied as a bone implant material. The aim of this work was synthesis and characterization of composite hydroxyapatite-chitosan through in situ way utilized by microwave irradiation with time variation. A composite which was formed by hydroxyapatite and 2% chitosan solution through in situ way were irradiated by 270-watt power for 20-60 minutes with an interval of 10 minutes. The XRD results showed peak hydroxyapatite and chitosan. Meanwhile, the highest crystallite size was found in 50 minutes composite with value 20.87 nm. The FTIR spectroscopy identified the functional group of hydroxyapatite and chitosan (NH). Increase in irradiation time caused reduction of chitosan-based on the existence of NH composition. Highest mass was present at time 20 minutes which means the reduction of mass also occurred with increasing irradiation time. The SEM and EDX results indicated the hydroxyapatite has filled chitosan matrix. The presence of NH cluster proved that microwave irradiation method can be used to synthesize nanocomposites hydroxyapatite and chitosan. Increase in irradiation time caused a reduction of chitosan existence and its mass.