The trans insertion-splicing (TIS) reaction is a technique that can be used to site-specifically insert an RNA donor substrate into a separate RNA acceptor substrate. The TIS reaction, which is catalyzed by a group I intron-derived ribozyme from Pneumocystis carinii, is described with regards to system design, ribozyme preparation, and the overall protocol for conducting the TIS reaction. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Dotson, P. P., Hart, J., Noe, C., & Testa, S. M. (2012). Ribozyme-mediated trans insertion-splicing into target RNAs. Methods in Molecular Biology, 848, 385–394. https://doi.org/10.1007/978-1-61779-545-9_24
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