The behavior of nanoparticles towards proteins is an important aspect across wide areas of nanotoxicology and nanomedicine. In this chapter, we describe a procedure to study the adsorption of proteins onto nanoparticle surfaces. Circular dichroism (CD) spectroscopy is utilized to quantify the amount of free protein in a solution, and the experimental information is evaluated to derive equilibrium constants for the protein adsorption/desorption equilibrium. These equilibrium constants are comparable parameters in describing the interactions between proteins and nanoparticles. © 2013 Springer Science+Business Media New York.
CITATION STYLE
Treuel, L., & Malissek, M. (2013). Interactions of nanoparticles with proteins: Determination of equilibrium constants. Methods in Molecular Biology, 991, 225–235. https://doi.org/10.1007/978-1-62703-336-7_21
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