Osteoclasts are bone-resorbing multinucleated cells derived from the monocyte–macrophage lineage. Bone-forming osteoblasts play a role in the formation of osteoclasts. Osteoblasts/stromal cells express two cytokines essential for osteoclastogenesis: receptor activator of nuclear factor κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Using RANKL and M-CSF, osteoclasts can be induced from monocyte–macrophage lineage cells even in the absence of osteoblasts. We describe here methods for the identification of osteoclasts formed in vitro.
CITATION STYLE
Udagawa, N., Yamashita, T., Kobayashi, Y., & Takahashi, N. (2011). Identification of Osteoclasts in Culture. In Methods in Molecular Biology (Vol. 690, pp. 273–284). Humana Press Inc. https://doi.org/10.1007/978-1-60761-962-8_18
Mendeley helps you to discover research relevant for your work.