Gas chromatography and gas chromatography-mass spectrometry analysis of Indonesian Croton tiglium seeds

Abstract

The result of phytochemical analysis showed that the hexane-soluble extract of seeds of C. tiglium contained fatty acids, terpenoids and alkaloids, while the ethanol-soluble extract of the seeds contained alkaloids, flavonoids, steroids, terpenoids and saponins. Moisture and proximate analysis showed that the seeds contained fat up to 40,1%, protein 26%, carbohydrate 15.51 and other elements such as fiber, moisture and ash. Gas Chromatography (GC) analysis on hexane-soluble extract of the seeds, using available instrument and reference-standards in our laboratory, showed 17 peaks indicating that at least the fat contained 17 compounds. Part of the compounds, 8 of them were identified as fatty acids and the 9 were unknown. The highest fatty acid level was linoleic acid (43.67%), oleic acid (19.98%) and myristic acid (7.64%). The Gas Chromatography-Mass Spectrometry (GC-MS) analysis of the hexane-soluble extract showed at least 32 compounds and the ethanol-soluble extract showed at least 25 compounds. © 2006 Asian Network for Scientific Information.