Purification and Characterisation of a Fungal beta-Mannanase.

Abstract

A mannanase from "Cellulase 36", a commercial preparation of Aspergillus enzymes, has been purified and characterised. The purified enzyme contains traces of cellulase and laminarinase activities. The purification factor is 61 and the enzyme yield 11 %. The molecular weight was determined and found to be 42 000 ± 2 000. The enzyme is rich in aromatic and acidic amino acids. A solution of guaran is partly broken down to mannobiose and mannotriose by the purified enzyme. The pH optima and stability as well as temperature optimum and stability have been determined.