Efficacy of Mosquito Repellent and Adulticidal Activities of Halophila Ovalis Extract Against Filaria Vectors

Abstract

The repellent and adulticidal activities were analyzed for ethanol extract of Halophila ovalis against Culex quinquefasciatus (Cx. quinquefasciatus). The extraction was done in soxhlet apparatus using ethanol as solvent. The repellent activity of the Halophila ovalis was determined against Culex quinquefasciatus in different concentration of the extract at 100, 150, 200, 250 and 300 μL/cm2. Adulticidal activity of the Halophila ovalis extract was tested against four to five day old female adults of Culex quinquefasciatus. The adult mortality was observed 24h under the laboratory conditions. Each experiment was conducted with the three replicates and a concurrent control group. As per the WHO guidelines, the experiments were conducted. At 250 μL of concentration extracts of Halophila ovalis showed maximum repellency percentage of 95% against Culex quinquefasciatus. The highest adulticidal activity was observed in 100 μL concentration against Culex quinquefasciatus with LC50 were (50.2 ± 0.7) μL/ml and (51.2 ± 0.9) μL/ml. The GC-MS reveals unique chemical compounds obtained from the extract of Halophila ovalis. The present study identifies the active insecticidal compounds from Halophila ovalis by GC-MS and from the results it can be concluded the extract of Halophila ovalis can be novel efficient biocontrol source against filariasis mosquitoes.